Zentrum für Biochemie und Molekularbiologie

Christoph Plieth

Am Botanischen Garten 9, R. ABG9/522
Telefon: +49 431 880-3888
Telefax: +49 431 880-4929

Affiliation:    Christian-Albrechts-Universität
Institute:      Zentrum für Biochemie und Molekularbiologie (BiMo)
Street:          Am Botanischen Garten 9 (Rm 522)
City:              D-24118 Kiel
Country:       Germany
Phone:         #49 (0)431 880 3888 (Office)
                      #49 (0)431 880 3887 (Lab#1)
                      #49 (0)431 880 3885 (Lab#2)
FAX:              #49 (0)431 880 4929
Email:         cplieth(at)bimo.uni-kiel.de
No responsibility is taken for the content of external web sites here referred to by a '[LINK]'.
Für den Inhalt von Seiten, auf die hier über einen '[LINK]' verwiesen wird, wird keine Verantwortung übernommen.


Research Area : Plant cellular stress response signalling and molecular biophysics


We are investigating cellular signalling events in response to abiotic environmental challenges in plants. Currently running projects focus on the assessment of indicator proteins usable to non-invasively quantify anions flux, pH changes, calcium release, and shifts of other cellular and subcellular parameters, relevant for cellular signal transduction.


Research Profile

Plant cell biology, Plant Physiology, Calcium Signaling, ROS signalling and antioxidative defense, cellular redox regulation, Membrane ion transport, abiotic stress response, salt stress, Cold response, Photosynthesis, Graviperception and gravitropism.


Concept & Idea:

Cellular signaling networks forming plant responses caused by abiotic stimuli or by environmental challenges.


Material & Methods:

Fluorescent proteins, GFP, luciferases, Aequorin, Recombinant Fusion Proteins, Fluorescein derivatives, transgenic reporter plants expressing fluorescent and luminescent indicator proteins, Molecular cloning, Recombinant protein expression, Protein purification, UV-VIS spectroscopy, Fluorescence microscopy and ratio imaging, FRET-Analysis, Bioluminescence, luminometry, time kinetics analyses, amperometric oxygen recording




'*' designates the corresponding author

Peer-reviewed Research Papers

Plieth, C.* 2019 "Peroxide-Induced Liberation of Iron from Heme Switches Catalysis during Luminol Reaction and Causes Loss of Light and Heterodyning of Luminescence Kinetics" ACS Omega 4(2):3268-3279; [LINK]  [PDF]

Plieth, C.* 2018 "Redox Modulators Determine Luminol Luminescence Generated by Porphyrin-Coordinated Iron and May Repress 'Suicide Inactivation' " ACS Omega 3(9):12295-12303; [LINK]   [PDF]

Morgan, S.H.; Lindberg, S.; Maity, P.J.; Geilfus, C.-M.; Plieth, C.; Mühling, K.-H.*; "Calcium improves apoplastic–cytosolic ion homeostasis in salt-stressed Vicia faba leaves" functional Plant Biology 44(5): 515-524;[LINK]  [PDF]

Plieth, C.* 2016  "Calcium, Metaphors, and Zeitgeist in Plant Sciences" Plant Physiology 171(3) 1790-1793 DOI: [LINK]   [PDF]

Geilfus, C.-M.*; Mühling, K.-H.; Kaiser, H.; Plieth, C. 2014 "Bacterially produced Pt-GFP as ratiometric dual-excitation sensor for in planta mapping of leaf apoplastic pH in intact Avena sativa and Vicia faba" Plant Methods   doi:10.1186/1746-4811-10-31 [LINK]   [PDF]

Schöler, A.; Zaharieva, I.; Zimmermann, S.; Wiechen, M.; Manke, A.-M.; Kurz, P.*; Plieth, C.; Dau, H. 2014 "Biogenic manganese-calcium oxides on the cell walls of the algae Chara corallina: elemental composition, atomic structure and water-oxidation catalysis" European Journal of Inorganic Chemistry  2014(4): 780-790  DOI: 10.1002/ejic.201300697  [LINK]   [PDF]

Saleh, L.*; Plieth, C. 2013 "A9C-sensitive Cl--accumulation in Arabidopsis thaliana root cells during salt stress is controlled by internal and external calcium"  Plant Signaling & Behavior  eLocation ID: e24259  [LINK]  [PDF]    [suppl]

Masood, S.; Saleh, L.; Witzel, K.; Plieth, C.; Mühling, K.-H.* 2012 "Determination of oxidative stress in wheat leaves as influenced by boron toxicity and NaCl stress" Plant Physiology & Biochemistry 56: 56-61 [LINK]   [PDF]

Plieth, C.* 2012 "Apoplastic calcium executes a shut-down function on plant peroxidases - a hypothesis" Plant Signaling & Behavior 7(6): 678-681 [LINK]    [PDF]

Plieth, C.*; Vollbehr, S. 2012 "Calcium promotes activity and confers heat stability on plant peroxidases" Plant Signaling & Behavior 7(6): 650-660 [LINK]   [PDF]

Saleh, L.; Plieth, C.* 2010 "Total low molecular weight antioxidants as a summary parameter, quantified in biological samples by a chemiluminescent inhibition assay" Nature Protocols 5(10): 1627-1634 [LINK]    [PDF]

Saleh, L.; Plieth, C.* 2010 "A coelenterazine-based luminescence assay to quantify high molecular weight superoxide anion scavenger activities" Nature Protocols 5(10): 1635-1641  [LINK]  [PDF]

Plieth, C.* 2010 "Signal percolation through plants and the shape of the calcium signature" Plant Signaling & Behavior 5(4): 379-385  [LINK]   [PDF]

Nagel-Volkmann, J.*; Plieth, C.; Becker, D.; Lüthen, H.; Dörffling, K. 2009 "Cold-induced cytosolic free calcium ion concentration changes in wheat" Journal of Plant Physiology 166(17): 1955-1960  [LINK]  [PDF]

Saleh, L.; Plieth, C.* 2009 "Fingerprinting antioxidative activities in plants" Plant Methods 5:2  doi:10.1186/1746-4811-5-2  [LINK]   [PDF]

Schulte, A.; Lorenzen, I.; Böttcher, M.; Plieth, C.* 2006  "A novel fluorescent pH probe for expression in plants"  Plant Methods 2:7 doi:10.1186/1746-4811-2-7  [LINK]  [PDF]

Plieth, C.* 2005 "Calcium: Just another regulator in the machinery of life?" Annals of Botany 96: 1-8  [LINK]     [PDF]

Hoffmann, A.; Hammes, E.; Plieth, C.; Desel, C.; Sattelmacher, B.; Hansen, U.-P.*  2005 "Effect of CO2 supply on formation of reactive oxygen species in Arabidopsis thaliana"  Protoplasma  227(1): 3-9 [PDF] 

Hammes, E.; Hoffmann, A.; Plieth, C.; Hansen, U.-P.*  2005  "Light-induced decrease in DCF fluorescence of wheat leaves in the presence of salicyl hydroxamate"  Protoplasma  227(1): 11-15  [PDF]   

Lorenzen, I., Aberle, T., Plieth, C.*  2004  "Salt stress-induced chloride flux: A study using transgenic Arabidopsis expressing a fluorescent anion probe"  The Plant Journal  38: 539-544 [LINK]    [PDF]

Gao, D.; Trewavas, A.J.; Knight, M.R.; Sattelmacher, B.; Plieth, C.*  2004  "Self-reporting Arabidopsis thaliana expressing pH- and [Ca2+]- indicators unveil ion dynamics in the cytoplasm and in the apoplast under abiotic stress."   Plant Physiology 134(3): 898-908 [LINK]      [PDF]

Plieth, C.*; Trewavas, A.J.  2002  "Reorientation of seedlings in the earth's gravitational field induces cytosolic calcium transients."  Plant Physiology  129(2): 786-796 [LINK]      [PDF]

Plieth, C.* 2001 "Plant calcium signaling and monitoring - pros and cons and recent experimental approaches - a review" Protoplasma 218: 1-23 [PDF] 

Plieth, C.*; Sattelmacher, B.; Knight, M.R.  2000  "Ammonia uptake and cellular alkalization in roots: The involvement of cytoplasmic calcium" Physiologia Plantarum  110: 518-523 [LINK]        [PDF]

Plieth, C.* 1999 "Temperature sensing by plants: Calcium permeable channels as primary sensors - a Model."  Journal of Membrane Biology  172: 121-127 [LINK]     [PDF]

Plieth, C.*; Sattelmacher, B.; Hansen, U.-P.; Knight, M.R.  1999  "Low pH-mediated elevations in cytosolic calcium are inhibited by aluminium: a potential mechanism for aluminium toxicity" The Plant Journal   18(6): 643-650 [LINK]     [PDF]

Plieth, C.*; Hansen, U.-P.; Knight, H.; Knight, M.R.  1999  "Temperature sensing by plants: The primary characteristics of signal perception and calcium response."  The Plant Journal  18(5): 491-497 [LINK]     [PDF]

Plieth, C.*, Sattelmacher, B.; Hansen, U.-P.; Thiel, G.  1998  "The action potential in Chara - Ca2+ release from internal stores"  The Plant Journal  13(2): 167-175 [LINK]     [PDF]

Bauer, C.S.; Plieth, C.; Hansen, U.-P.; Simonis, W.; Schönknecht, G.*  1998  "A steep Ca2+-dependence of a K+ channel in a unicellular green alga"   Journal of Experimental Botany   327(49): 1761-1765 [LINK]     [PDF]

Plieth, C., Hansen, U.-P.*   1998  "Cytoplasmic Ca2+ and H+ buffers in green algae: a reply"  Protoplasma   203: 210-213 [LINK]     [PDF]

Plieth, C., Sattelmacher, B., Hansen, U.-P.*  1998  "Light-induced Ca2+-transients in green Plants II: The effect on the K+-channel as studied by kinetic analysis in Chara corallina"  Planta   207: 52-59 [LINK] [PDF]

Plieth, C., Sattelmacher, B., Hansen, U.-P.*  1998  "Light-induced Ca2+-transients in green Plants I: Methodological aspects of chlorotetracycline usage in algae and higher-plant cells"  Planta   207: 42-51 [LINK] [PDF]

Bauer, C.S.; Plieth, C.; Bethmann, B.; Popescu, O.; Hansen, U.-P.; Sattelmacher, B.; Simonis, W.; Schönknecht, G.*  1998  "Strontium-induced repetitive Calcium spikes in a unicellular green alga" Plant Physiology  117: 545-557 [LINK]       [PDF]

Thiel, G.*, Homann, U., Plieth, C.  1997  "Ion channel activity during the action potential in Chara: a new insight with new techniques" Journal of Experimental Botany  48: 609-622 [LINK]    [PDF]

Plieth, C., Sattelmacher, B., Hansen, U.-P.*   1997  "Cytoplasmic Ca2+-H+-exchange buffers in green algae"  Protoplasma   198: 107-124 [LINK]   [PDF_ALL]

Bauer, C., Plieth, C., Hansen, U.-P., Sattelmacher, B., Simonis, W., Schönknecht, G.*  1997  "Repetitive Ca2+ spikes in an unicellular alga"  FEBS Letters   405: 390-393 [LINK]   [PDF]

Plieth, C., Hansen, U.-P.* 1996  "Methodological aspects of pressure loading of Fura-2 into Characean cells"  Journal of Experimental Botany   47: 1601-1612 [LINK] [PDF] 

Mühling, K.-H.*, Plieth, C., Hansen, U.-P.; Sattelmacher, B.  1995  "Apoplastic pH of intact leaves of Vicia faba as influenced by light"  Journal of Experimental Botany  46:  377-382 [LINK] [PDF]

Plieth, C.*, Tabrizi, H., Hansen, U.-P.  1994  "Relationship between banding and photosynthetic activity in Chara corallina as studied by the spatial different induction curves of chlorophyll fluorescence observed by an image analysis system"  Physiologia Plantarum  91:  205-211 [LINK] [PDF]  

Plieth, C.*, Hansen, U.-P.  1992  "Light dependence of protoplasmic streaming in Nitella flexilis L. as measured by means of laser velocimetry"  Planta  188:  332-339 [LINK] [PDF] 


Books and solicited Articles (Essays, Reports, Book Chapters, Non-peer-reviewed Articles)

Plieth, C.* 2020 " 'Ehre wem Ehre gebührt' - Ist der Dr. h.c. noch zeitgemäß?" Laborjournal 7/8: 46-49 [LINK]   

Plieth, C.*, Gao, D., Knight, M.R., Trewavas, A.J.; Sattelmacher, B. 2007 "Self-reporting Arabidopsis thaliana expressing pH- and [Ca2+]- indicators unveil apoplastic ion dynamics" In: Sattelmacher B., Horst W. (Eds.) "The apoplast of higher plants: compartment of storage, transport and reactions." Kluwer Academic Publishers - ISBN 9781402058424 pp. 373-392 [LINK]  [PDF]  

Plieth, C.*  2006  "Aequorin as a reporter gene"   Methods in Molecular Biology 323: 307-327 In: Salinas J.; Sanchez-Serrano J.J. (Eds.) "Arabidopsis Protocols" 2nd Edition, Humana Press - ISBN 1-58829-395-5 [LINK]  [PDF]

Schoeler, A., Liebisch, P. Müller, C., Plieth, C., Erko, A., Dau, H.* 2004 "Comparative XAS study of synthetic manganese oxides and biogenic deposits on the cell-wall of the green alga Chara corallina" BESSY Annual Report 2004: 449–451 [LINK]   [PDF] 

Plieth, C.*  2004  "Ion signalling in plants probed by genetically encoded indicators" Cumulative Script 'State Doctorate' for obtaining The Postdoctoral Lecture Qualification (Habilitation); Zentrum für Biochemie und Molekularbiologie in der Mathematisch-Naturwissenschaftlichen Fakultät der Christian-Albrechts-Universität zu Kiel; pp.1-167[PDF]  

Plieth, C.* 2003 "Self-reporting transgenic plants shed new light on cellular signal transduction pathways" In: Temps F. (Ed.) "Chemistry in Kiel ('Bunsentagung 2003' Supplement)" VMK-Verlag, Monsheim, Germany pp.99-104[PDF] 

Plieth, C.*, Sattelmacher B., Trewavas A.J., Hansen, U.-P., Knight M.R.  2001  "Engineering plants expressing calcium and pH indicators in the cytoplasm and the apoplast." in Horst (Ed.) "Plant Nutrition - Food security and sustainability of agro-ecosystems through basic and applied research (Proceedings of XIV International Plant Nutrition Colloquium, Hannover, Germany, 2001) - Plant and Soil Science Series" Kluwer Academic Publishers, Dordrecht, Netherlands - ISBN 9781402010569 pp.252-253 [LINK]   

Fricker, M.D.*, Parsons, A., Tlalka, M., Blancaflor, E., Gilroy, S., Meyer, A., Plieth, C. 2001 "Fluorescent probes for living plant cells." In: C. Hawes, and B. Satiat-Jeunemaitre (Ed.) "Plant Cell Biology: A Practical Approach" Oxford University Press 2nd Edition, Oxford - ISBN 9780199638666 pp. 35-84. [PDF]

Fricker, M.D.*; Plieth, C.; Knight, H.; Blancaflor, E.; Knight, M.R.; White, N.; Gilroy, S.  1998  "Fluorescence and luminescence techniques to probe ion activities in living plant cells"  in: Mason, B. (Ed.)  "Fluorescent and luminescent probes for biological activity"  Academic Press - ISBN 9780124478367 pp.569-596 [PDF] 

Plieth, C.*  1996  "Recording ion concentrations and their transients in green plant cells and tissues using fluorescence ratio imaging microscopy and laser-Doppler velocimetry“  Doctoral Thesis (Dissertation - German text), Institut für Angewandte Physik der Christian-Albrechts-Universität zu Kiel; pp.1-283  © 1996 Shaker-Verlag Aachen - ISBN 3-8265-1399-1  [PDF]

Plieth, C.*, Moldaenke, C.  1995  "Biomonitoring: Methoden zur kontinuierlichen Gewässerüberwachung - Teil 2: Gewässerbiomonitoring mit Algen und Mikroorganismen" (German text) WasserAbwasserPraxis  Bertelsmann Fachzeitschriften, Gütersloh  4 (2):  22-28. [PDF]

Plieth, C.*  1995  "Biomonitoring: Methoden zur kontinuierlichen Gewässerüberwachung - Teil 1: Gewässerbiomonitoring mit Fischen, Kleinkrebsen und Muscheln" (German text) WasserAbwasserPraxis  Bertelsmann Fachzeitschriften, Gütersloh  4 (1):  20-24. [PDF]

Plieth, C.*  1991  "Biosensors - State-of-the-art, Techniques, Applications and Chances for Companies in Schleswig-Holstein"  (German text: "Biosensoren - Studie zum Stand der Technik im Biosonden-/-sensorenbereich und Perspektiven für Schleswig-Holsteinische Firmen") published by Ministerium für Natur, Umwelt und Landesentwicklung (MNUL) des Landes Schleswig-Holstein, Kiel   pp.  1-218.  [PDF]



Plieth, C.*  2004  "Luminescence-Measuring-Chamber" AZ-Nr. 103 18 994.7 - B3 2004.03.11 (Deutsches Patent- und Markenamt, München) Patent owner: Christian-Albrechts-Universität, Kiel  [PDF]    (expired)

Plieth, C.*  2011  "Solid phase biofilm reactor for biomass production of adhesive cells"  (Deutsches Patent- und Markenamt, München) Patent owner: Christian-Albrechts-Universität, Kiel    [LINK]  (withdrawn)



Professional Career






Practical courses:              

Basic practical "Biochemistry and Molecular Biology" (Bc bcmb-102)

Advanced practical "Molecular Biology for biochemistry students" (Ms bcmb-271)

Lab-Techniques and -Methods Part: 'Chromatography' (Bs biol-101)



"Biochemistry and Molecular Biology III (Functions)" (Ms bcmb-203)

"Biochemistry and Molecular Biology IV (Methods)" (Ms bcmb-104)
Lab-Techniques and -Methods Part: 'Chromatography' (Bs biol-101)

"Molecular Biophysics" (Ms bcmb-152)



"Biochemistry and Molecular Biology (Basics)" (Bc bcmb-102)
"Biochemistry and Molecular Biology III (Functions)" (Ms bcmb-203)

"Biochemistry and Molecular Biology IV (Methods)" (Ms bcmb-104)



Students-Lab (Ms bcmb-285)

Bachelor Theses
Master Theses