Zentrum für Biochemie und Molekularbiologie



  • Krossa, S., Scheidig, A.J., Grötzinger, J., Lorenzen, I. (2018). Redundancy of protein disulfide isomerases in the catalysis of the inactivating disulfide switch in A Disintegrin and Metalloprotease 17. Sci Rep. 8:1103. doi: 10.1038/s41598-018-19429-4.


  • Schäfer, M., Granato, D.C., Krossa, S., Bartels, A.K., Yokoo, S., Düsterhöft, S., Koudelka, T., Scheidig, A.J., Tholey, A., Paes Leme, A.F., Grötzinger, J. and Lorenzen, I. (2017) GRP78 protects a disintegrin and metalloprotease 17 against protein-disulfide isomerase A6 catalyzed inactivation. FEBS Lett 591: 3567-3587.
  • Lorenzen, I., Mullen, L., Bekeschus, S. and Hanschmann, E.M. (2017) Redox Regulation of Inflammatory Processes Is Enzymatically Controlled. Oxid Med Cell Longev 2017: 8459402. (Review)
  • Grötzinger, J., Lorenzen, I. and Düsterhöft, S. (2017) Molecular insights into the multilayered regulation of ADAM17: The role of the extracellular region. Biochim Biophys Acta 1864: 2088-2095. (Review)
  • Riethmueller, S., P. Somasundaram, J.C. Ehlers, C.W. Hung, C.M. Flynn, J. Lokau, M. Agthe, S. Düsterhöft, Y. Zhu, J. Grötzinger, I. Lorenzen, T. Koudelka, K. Yamamoto, U. Pickhinke, R. Wichert, C. Becker-Pauly, M. Radisch, A. Albrecht, M. Hessefort, D. Stahnke, C. Unverzagt, S. Rose-John, A. Tholey, and C. Garbers (2017) Proteolytic Origin of the Soluble Human IL-6R In Vivo and a Decisive Role of N-Glycosylation. PLoS Biol. 15(1): p. e2000080.


  • Groth, E., J. Pruessmeyer, A. Babendreyer, J. Schumacher, T. Pasqualon, D. Dreymueller, S. Higashiyama, I. Lorenzen, J. Grötzinger, D. Cataldo, and A. Ludwig (2016) Stimulated release and functional activity of surface expressed metalloproteinase ADAM17 in exosomes. Biochim Biophys Acta. 1863(11): p. 2795.
  • Lorenzen, I., J. Lokau, Y. Korpys, M. Oldefest, C.M. Flynn, U. Künzel, C. Garbers, M. Freeman, J. Grötzinger, and S. Düsterhöft (2016) Control of ADAM17 activity by regulation of its cellular localisation. Sci Rep. 6: p. 35067.
  • Sommer, A., F. Kordowski, J. Buch, T. Maretzky, A. Evers, J. Andra, S. Düsterhöft, M. Michalek, I. Lorenzen, P. Somasundaram, A. Tholey, F.D. Sönnichsen, K. Kunzelmann, L. Heinbockel, C. Nehls, T. Gutsmann, J. Grötzinger, S. Bhakdi, and K. Reiss (2016) Phosphatidylserine exposure is required for ADAM17 sheddase function. Nat Commun. 7: p. 11523.


  • Düsterhöft, S., M. Michalek, F. Kordowski, M. Oldefest, A. Sommer, J. Roseler, K. Reiss, J. Grötzinger, and I. Lorenzen (2015) Extracellular Juxtamembrane Segment of ADAM17 Interacts with Membranes and Is Essential for Its Shedding Activity. Biochemistry. 54(38): p. 5791.
  • Oldefest, M., S. Düsterhöft, C. Desel, S. Thysen, C. Fink, B. Rabe, R. Lories, J. Grötzinger, and I. Lorenzen (2015) Secreted Frizzled-related protein 3 (sFRP3)-mediated suppression of interleukin-6 receptor release by A disintegrin and metalloprotease 17 (ADAM17) is abrogated in the osteoarthritis-associated rare double variant of sFRP3. Biochem J. 468(3): p. 507.


  • Bungeroth, M., S. Appenzeller, A. Regulin, W. Volker, I. Lorenzen, J. Grotzinger, M. Pendziwiat, and G. Kuhlenbaumer (2014) Differential aggregation properties of alpha-synuclein isoforms. Neurobiol Aging. 35(8):1913-9.
  • Düsterhöft, S., K. Höbel, M. Oldefest, J. Lokau, G.H. Waetzig, A. Chalaris, C. Garbers, J. Scheller, S. Rose-John, I. Lorenzen, and J. Grötzinger (2014) A Disintegrin and Metalloprotease 17 Dynamic Interaction Sequence, the Sweet Tooth for the Human Interleukin 6 Receptor. J Biol Chem. 289(23): p. 16336.
  • Meyer, C., K. Berg, K. Eydeler-Haeder, I. Lorenzen, J. Grötzinger, S. Rose-John, and U. Hahn (2014) Stabilized Interleukin-6 receptor binding RNA aptamers. RNA Biol. 11(1).


  • Düsterhöft, S., S. Jung, C.W. Hung, A. Tholey, F.D. Sönnichsen, J. Grötzinger, and I. Lorenzen (2013) Membrane-proximal domain of a disintegrin and metalloprotease-17 represents the putative molecular switch of its shedding activity operated by protein-disulfide isomerase. J Am Chem Soc. 135(15): p. 5776.
  • Garbers, C., B. Spudy, S. Aparicio-Siegmund, G.H. Waetzig, J. Sommer, C. Holscher, S. Rose-John, J. Grotzinger, I. Lorenzen, and J. Scheller (2013) An interleukin-6 receptor-dependent molecular switch mediates signal transduction of the IL-27 cytokine subunit p28 (IL-30) via a gp130 protein receptor homodimer. J Biol Chem. 288(6): p. 4346.
  • Michalek, M., F.D. Sönnichsen, R. Wechselberger, A.J. Dingley, C.W. Hung, A. Kopp, H. Wienk, M. Simanski, R. Herbst, I. Lorenzen, F. Marciano-Cabral, C. Gelhaus, T. Gutsmann, A. Tholey, J. Grötzinger, and M. Leippe (2013) Structure and function of a unique pore-forming protein from a pathogenic acanthamoeba. Nat Chem Biol. 9(1): p. 37.
  • Oldefest, M., J. Nowinski, C.W. Hung, D. Neelsen, A. Trad, A. Tholey, J. Grötzinger, and I. Lorenzen (2013) Upd3 - An ancestor of the four-helix bundle cytokines. Biochem Biophys Res Commun. 436(1): p. 66.
  • Trad, A., H.P. Hansen, M. Shomali, M. Peipp, K. Klausz, N. Hedemann, K. Yamamoto, A. Mauermann, C. Desel, I. Lorenzen, H. Lemke, S. Rose-John, and J. Grötzinger (2013) ADAM17-overexpressing breast cancer cells selectively targeted by antibody-toxin conjugates. Cancer Immunol Immunother. 62(3): p. 411.
  • Trad, A., M. Riese, M. Shomali, N. Hedeman, T. Effenberger, J. Grötzinger, and I. Lorenzen (2013) The disintegrin domain of ADAM17 antagonises fibroblastcarcinoma cell interactions. Int J Oncol. 42(5): p. 1793.
  • Lorenzen, I., J. Lokau, S. Düsterhoft, A. Trad, C. Garbers, J. Scheller, S. Rose-John, and J. Grötzinger (2012) The membrane-proximal domain of A Disintegrin and Metalloprotease 17 (ADAM17) is responsible for recognition of the interleukin-6 receptor and interleukin-1 receptor II. FEBS Lett. 586(8): p. 1093.


  • Magbanua, E., T. Zivkovic, B. Hansen, N. Beschorner, C. Meyer, I. Lorenzen, J. Grötzinger, J. Hauber, A.E. Torda, G. Mayer, S. Rose-John, and U. Hahn (2012) d(GGGT) 4 and r(GGGU) 4 are both HIV-1 inhibitors and interleukin-6 receptor aptamers. RNA Biol. 10(2).
  • Meyer, C., K. Eydeler, E. Magbanua, T. Zivkovic, N. Piganeau, I. Lorenzen, J. Grötzinger, G. Mayer, S. Rose-John, and U. Hahn (2012) Interleukin-6 receptor specific RNA aptamers for cargo delivery into target cells. RNA Biol. 9(1): p. 67.
  • Yamamoto, K., A. Trad, A. Baumgart, L. Huske, I. Lorenzen, A. Chalaris, J. Grötzinger, T. Dechow, J. Scheller, and S. Rose-John (2012) A novel bispecific single-chain antibody for ADAM17 and CD3 induces T-cell-mediated lysis of prostate cancer cells. Biochem J. 445(1): p. 135.


  • Garbers, C., W. Thaiss, G.W. Jones, G.H. Waetzig, I. Lorenzen, F. Guilhot, R. Lissilaa, W.G. Ferlin, J. Grötzinger, S.A. Jones, S. Rose-John, and J. Scheller (2011) Inhibition of classic signaling is a novel function of soluble glycoprotein 130 (sgp130), which is controlled by the ratio of interleukin 6 and soluble interleukin 6 receptor. J Biol Chem. 286(50): p. 42959.
  • Jung, S., J. Mysliwy, B. Spudy, I. Lorenzen, K. Reiss, C. Gelhaus, R. Podschun, M. Leippe, and J. Grötzinger (2011) Human beta-defensin 2 and beta-defensin 3 chimeric peptides reveal the structural basis of the pathogen specificity of their parent molecules. Antimicrob Agents Chemother. 55(3): p. 954.
  • Lorenzen, I., W. Shang, M. Perbandt, M.V. Petoukhov, D.I. Svergun, G.H. Waetzig, S. Rose-John, R. Hilgenfeld, and J. Grötzinger (2011) The structure of the unliganded extracellular domain of the interleukin-6 signal transducer gp130 in solution. Eur J Cell Biol. 90(6-7): p. 515.
  • Lorenzen, I., A. Trad, and J. Grötzinger (2011) Multimerisation of A disintegrin and metalloprotease protein-17 (ADAM17) is mediated by its EGF-like domain. Biochem Biophys Res Commun. 415(2): p. 330.
  • Trad, A., N. Hedemann, M. Shomali, V. Pawlak, J. Grötzinger, and I. Lorenzen (2011) Development of sandwich ELISA for detection and quantification of human and murine a disintegrin and metalloproteinase17. J Immunol Methods. 371(1-2): p. 91.


  • Mysliwy, J., A.J. Dingley, M. Stanisak, S. Jung, I. Lorenzen, T. Roeder, M. Leippe, and J. Grötzinger (2010) Caenopore-5: the three-dimensional structure of an antimicrobial protein from Caenorhabditis elegans. Dev Comp Immunol. 34(3): p. 323.
  • Suthaus, J., A. Tillmann, I. Lorenzen, E. Bulanova, S. Rose-John, and J. Scheller (2010) Forced homo- and heterodimerization of all gp130-type receptor complexes leads to constitutive ligand-independent signaling and cytokine-independent growth. Mol Biol Cell. 21(15): p. 2797.


  • Egli, R.J., L. Southam, J.M. Wilkins, I. Lorenzen, M. Pombo-Suarez, A. Gonzalez, A. Carr, K. Chapman, and J. Loughlin (2009) Functional analysis of the osteoarthritis susceptibility-associated GDF5 regulatory polymorphism. Arthritis Rheum. 60(7): p. 2055.
  • Dingley, A.J., I. Lorenzen, and J. Grötzinger (2008) NMR analysis of viral protein structures. Methods Mol Biol. 451: p. 441.
  • Schulte, A., I. Lorenzen, M. Bottcher, and C. Plieth (2006) A novel fluorescent pH probe for expression in plants. Plant Methods. 2: p. 7.
  • Mortier, E., A. Quemener, P. Vusio, I. Lorenzen, Y. Boublik, J. Grötzinger, A. Plet, and Y. Jacques (2006) Soluble interleukin-15 receptor alpha (IL-15R alpha)-sushi as a selective and potent agonist of IL-15 action through IL-15R beta/gamma. Hyperagonist IL-15 x IL-15R alpha fusion proteins. J Biol Chem. 281(3): p. 1612.
  • Lorenzen, I., A.J. Dingley, Y. Jacques, and J. Grötzinger (2006) The structure of the interleukin-15 alpha receptor and its implications for ligand binding. J Biol Chem. 281(10): p. 6642.
  • Lorenzen, I., T. Aberle, and C. Plieth (2004) Salt stress-induced chloride flux: a study using transgenic Arabidopsis expressing a fluorescent anion probe. Plant J. 38(3): p. 539.